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KMID : 0545120110210080796
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 8 p.796 ~ p.801
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Characterization of Novel Plasmid p1B146 from Corynebacterium tuberculostearicum
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Lukasz Wieteska
Eligia M. Szewczyk
Janusz Szemraj
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Abstract
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Corynebacterium tuberculostearicum B146, a strain derived from healthy human skin, contains a medium copy plasmid, p1B146. This plasmid was cloned and its complete nucleotide sequence determined. As a result, p1B146 was found to be 4,2 kb in size with a 53% G+C content, plus six open reading frames (ORFs) were distinguished. According to a computer-assisted alignment, two of the ORFs exhibited significant similarities to already-known common plasmid proteins, the first being the RepA gene, responsible for plasmid replication via a rolling-circle mechanism, and the second being an FtsK-like protein, the function of which remains unclear. The presence and quantity of RNA fragments in the putative ORFs were also evaluated.
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KEYWORD
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Corynebacterium, rolling-circle mechanism, FtsK-like protein, Plasmid
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